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Thereafter, lungs were either frozen to isolate RNA, inflation-fixed with paraformaldehyde for histology or lavaged to collect bronchoalveolar lavage fluid ( RNA ANALYSIS Total RNA was isolated from frozen lung tissue, quantified by spectrophotometry and assayed for messenger RNA transcript abundance using standard real time PCR methodology as described ( HISTOLOGY AND CYTOLOGY Standard techniques were used to fix lung tissue in 4% paraformaldehyde and to embed in paraffin

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Then, the main focus is maintaining vessel function upon the first innate reperfusion (as evidenced by an initial innate recovery while the blood supply is deprived) in IC rats [which is also applicable much later with additional bowel obstruction (IC + OB rats)], and subsequently upon massive reperfusion following the removal of vascular obstruction(s) (IC + RL rats)