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In the context of chronic disease, IL-1 levels are increased in the lungs of neutrophilic asthmatic mice and neutrophilic asthmatic patients, which may lead to impaired epithelial barrier function and increased mucus production, exacerbating inflammation 68

Age-adjusted metabolite analysis To assess whether age-related variation influenced the observed metabolomic alterations, an analysis of covariance (ANCOVA) was performed using age as a covariate

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