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The free-energy landscape built with the first two eigenvectors of the PCA analysis showed a less rugged surface and a smaller number of energy minima in the holo-form, in contrast with the behavior of the apo-form (Fig 2)

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We built a pseudo-temporal object using the imported Seurat data, performed dimensionality reduction on cells using the DDRTree method, and then used the dimensionality reduction function to determine the type of cell differentiation state