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The use of advanced techniques, such as computational mutagenesis, has been instrumental in identifying mutations that affect protein stability, which is crucial for understanding the underlying genetic causes of the deficiency

[12] The oral upper intake levels established for dietary copper do not translate directly to a copper-carrying peptide delivered subcutaneously, which bypasses the intestinal regulation of copper absorption entirely one more reason the systemic dosing of GHK-Cu is genuinely uncharacterized in humans