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Nevertheless, gliotoxin exhibited cytotoxicity to human umbilical vein endothelial cells (HUVECs) with concentrations higher than 500 nM [56,62], whereas there was no obvious cytotoxicity with concentrations lower than 300 nM, which is consistent with a previous study that found that gliotoxin induces apoptosis in activated human hepatic stellate cells with concentrations ranged from 0.3 to 7.5 M [63]