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the lysate was clarified by centrifugation at 15,000 g for 15 minutes, protein concentrations were determined using the BCA total protein assay, and then appropriate volumes of the lysates were diluted with 2 reducing Laemmli buffer and heated for ten minutes at 95 C

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I have diligently performed eccentric loading for the patellar tendinosis for months now and also reintroduced eccentric loading to the right forearm extensor since the 2nd-3rd week of the therapy