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Conclusions The highest chance of a clinical pregnancy or live birth occurred with -hCG levels of 253 IU/L (92.7% chance of clinical pregnancy and 74.5% chance of a live birth, at 13dp3dt or 11dp5dt)

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Under reaction conditions of pH 5, 1 h and 80 C, RCYs were determined to be 100 0.0%, 91.6 0.4%, 72.2 6.3%, 43.3 10.1% and 3.9 2.7% ( n = 3) for ligand concentrations of 10 4 , 10 5 , 10 6 , 10 7 , and 10 8 M, respectively

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