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10.1128/AEM.02942-09 Pedersen MB, Gaudu P, Lechardeur D, Petit MA, Gruss A: Respiration pathways in fermentative lactic acid bacteria and uses in biotechnology
Prior to any procedures, animals are anesthetized using a combination of xylazine (10 mg/kg) and ketamine (90 mg/kg) for up to one hour via the intraperitoneal route
As for safety, tolerable upper intake levels (known as ULs) are set for vitamins and minerals when evidence is sufficient