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Citric acid, a central intermediate in the tricarboxylic acid (TCA) cycle, has demonstrated glucose-lowering effects in animal studies, significantly reducing blood glucose and insulin resistance while enhancing insulin sensitivity (84)

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(2) V3-V4 Amplification Target regions were amplified with barcoded primers 341 F (5-CCTAYGGGRBGCASCAG-3) and 806 R (5-GGACTACNNGGGTATCTAAT-3) using Phusion High-Fidelity PCR Master Mix (New England Biolabs, USA)