6 Challenges and limitations 6.1 Safety and toxicity of nanoparticles Although nanocarriers represent highly effective platforms for brain-directed delivery and facilitate BBB traversal, several critical challenges remain
Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min
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The authors consider this phenomenon as a green biosynthesis of nanoparticles , and biological techniques that exploit plant cells and micro-organisms and for the biological production of metal sulphide nanoparticles are currently evaluated as an economically competitive and environmentally benign alternative to chemical processes [169,170,171,172,173]
