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Nimmo and Philip Cohen reported purification of protein phosphatase inhibitor-1 (PPI-1) from rabbit skeletal muscle utilizing an approach that includes heat treatment at 90 C as one of the purification steps (Nimmo and Cohen, 1978)

To distinguish between monomeric and assembled states, we determined the critical aggregation concentrations of compounds 1 and 2 to be 59.4 M and 114.7 M, respectively (Supplementary Fig

After measurement of OD 600 , bacterial cultures were adapted to 0.5 McFarland units (0.5 McFarland = OD 600 ) of a solution comprising 99.5 ml 0.36 N H 2 SO 4 + 0.5 ml 0.048 M BaCl 2 2H 2 O, OD 600 = 0.15

Active malignancy generally precludes GH optimization approaches