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In order to display the implications of GST for individual patients, a generalized additive model (GAM) (with spline) incorporating the subject-specific (longitudinal) random effects was plotted and adjusted for other clinical parameters to predict the outcomes 28,29

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The distance between the contour lines represents the steepness of the slope, the lesser the distance, the more is the steepness (changing pattern of interaction), while the larger space between contour lines represents a soft slope (lesser changing pattern) while no lines show flat region (constant type of interaction)

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