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Ghiron published a comprehensive study utilizing acrylamide as quencher and showed that this approach is very discriminating in sensing the degree of exposure of tryptophan residue in proteins, including a randomly coiled peptide adrenocorticotropin (Eftink and Ghiron, 1976)

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[55] If nervous system damage is present and blood testing is inconclusive, a lumbar puncture may be carried out to measure cerebrospinal fluid B 12 levels

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