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Primer sequences were: Sirt3 forward: ACAGCTACATGCACGGTCTG, reverse: ACACAATGTCGGGTTTCACA p16 forward: CGCAGGTTCTTGGTCACTGT, reverse: TGTTCACGAAAGCCAGAGCG Actb forward: GCCTTCCTTCTTGGGTATGG, reverse: AGCACTGTGTTGGCATAGAG

Ophiopogonin D improves osteointegration of titanium alloy implants under diabetic conditions by inhibition of ROS overproduction via Wnt/-catenin signaling pathway

The neutralized extract was then reacted with 5,5-dithiobis(2-nitrobenzoic acid) (DTNB), followed by reduction with nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of GR

(PubMed) Tanaka S, Haruma K, Kunihiro M, et al