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Observations were made in the morning, afternoon and evening of each day during the culture period to record the food intake, activity and mortality of the bullfrogs

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DNA constructs and mutagenesis PCR-amplified human SLC2A1 , DHHC9 , and GCP16 were subcloned into baculoviral vector pFastBac1, lentiviral vector pCDH (hygromycin resistance), bicistronic expression vector pIRES, or green fluorescent protein vector pEGFP-C1

Reconstitution note: NAD+ is acidic, and that acidity is the main driver of injection-site sting