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[138] developed an unlabeled combinatorial alanine affinity selection platform to promote the optimization of PPI modulators based on peptides, in which the i , i + 4 perfluoroaryl stapling strategy was applied to three highest frequency alanine-substituted sites of peptides and contributed to the formation of three highest affinity stapled peptides monitored by biolayer interferometry (BLI) competition assays

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doi: 10.1167/iovs.10-6235