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To assess whether RT probe binds to any proteins or reacts with free cysteine residues in some proteins that may interfere with the final ratio readouts, we incubated RT with cell lysate, which was dialyzed using a 3 K cut-off membrane to remove all small molecules including GSH, and observed no significant changes in fluorescent signal ratios even at the highest lysate concentration shown (protein concentration is 36 mg ml 1 based on a BCA assay), suggesting that there is no non-specific interactions or reactions between cellular proteins and RT which could significantly change the ratio readouts (Fig

How to Reduce Injection Reactions Rotate injection sites consistently Allow peptides to reach room temperature before injecting Inject slowly using a fine-gauge needle Avoid rubbing the site after injection Split larger doses into multiple sites if needed Some patients experience fewer reactions with intramuscular injections

Side effects are generally mild and may include temporary redness or swelling at the injection site, nausea, or mild headaches

References Kanner, L