The study addressed two questions: whether the fragment retained activity when delivered enterally rather than parenterally, and whether the effects observed in rodent models translated to human adipose tissue at the cellular level
The following day, cells were washed three times with DPBS and incubated with the corresponding secondary antibodies conjugated to Alexa Fluor 488 or Alexa Fluor 555 fluorophores (Thermo Fisher Scientific, Inc.) for 2 h at room temperature
LC contents of the extracellular fluid, liver, skeletal muscle, and kidney were 0.5, 1.3, 1.27, and 0.2 mmol/L, in that order [6]
Inhibition of cysteine uptake by p53 can sensitize cells to ferroptosis through downregulating the expression of SLC7A11 and GPX4, reduction of antioxidant capacity, and resultant ROS accumulation [53, 54]