10.2174/1381612823666170220163219 75 SikiricP.SeiwerthS.RucmanR.KolencD.VuleticL
Mitochondrial respiration rates (pmols 1 ) were normalized for mitochondrial suspension protein levels (pmols 1 g 1 ) in the isolated mitochondria preparation using the Pierce 660 nm protein assay (Thermo Scientific) to obtain the intrinsic mitochondrial respiration, which is an index of mitochondrial quality
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While enzymes such as catalase (EC 1.11.1.6), glutathione peroxidase (GPX, EC 1.11.1.19), and SOD are considered the first line of enzymatic antioxidant defense, other enzymes, such as glutathione reductase (GR, EC 1.6.4.2), glutathione S-transferase (GST, EC 2.5.1.18), and thioredoxin reductase (EC 1.6.4.5) [1,2,5,10], also contribute to defense