Cytotoxicity assays conducted on 3T3 fibroblasts, C2C12 myotubes, PC12 neurons, and RAW macrophages, demonstrated that neither Au nanospheres nor Au-PX-PLGA (0.010.5 mg/mL) caused significant toxicity, with cell viability remaining above 95%
This inertness is maintained by the highly acidic C-terminal tail, which plays a crucial role in preserving Syn in a soluble state 5,7,8
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