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Physico-chemical characterization of nanocarriers The average size, polydispersity index (PDI) and surface charge (zeta potential) of nanocarriers were measured by dynamic light scattering (Malvern Zetasizer Nano ZS, equipped with a HeNe laser ( = 632.8 nm), Malvern Instruments, UK)

Moreover, MDA and 4-HNE protein adducts were also identified in OA tissue sections by using immunohistochemical methods [47]

On the other hand, vitamins A and E are very important among antioxidants

In Vitro study in human lymphocytes showed its potent anti-oxidant potential as well as its abilities to reduce DNA damage and chromosome breakage induced by chemicals (51)