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Glutamate ligands and BPAM344 compounds were extracted from the cryo-EM structure (PDB code 9B36) and manually fitted into the corresponding densities in Coot

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These differ in their suitability for specific tissues, the extent to which tissue samples may be expanded in culture, the degree to which cultures reflect tissue organization and differentiation, the length of time that cultures may be maintained before passage and the cost of the required media (Fig