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pneumoniae in host cells To assess levofloxacin persistence of pneumococci, A549 and RAW 264.7 cells infected with pneumococci (as described above) were maintained in DMEM supplemented with 1% FBS and 6 mg/mL levofloxacin, while PLB-985 and PLB-985 nox2 -KO infected cells were cultured in RPMI 1640 with 1% FBS, 1.3% DMSO, and 6 mg/mL levofloxacin

Vaughan et al

Acknowledgements This project was supported by the National Natural Science Foundation of China (82272232, 82170775, 82370765, 82270802, 82100806, and 82300766) and the Open Project of Hubei Key Laboratory (2023KFZZ009)

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