Lysates were mixed 1:1 (T4:9, or T4:76, or T4:TuIb) and incubated with glutathione Sepharose, then the resin was intensively washed and phages were sequentially eluted with a glutathione buffer
It is composed of different layers that are fundamental to protect the eye and to maintain transparency
/ Water is necessary for the detoxification processes that glutathione supports
In brief, 0.2 L urine samples were analyzed by SDS-PAGE (10% gels) under reducing or non-reducing conditions and then transferred to polyvinylidene fluoride [PVDF (Immobilon -P Transfer Membrane, Merk Millipore Ltd., Burlington, MA, United States)] membranes by wet blotting (Mini Trans-Blot Electrophoretic Transfer Cell, BioRad Laboratories, Inc., Hercules, CA, United States)