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Collect cells by centrifugation at 200 g for 10 min at 4C

Similarly, coronary artery disease patients with single, double, or triple-vessel stenosis and patients with acute coronary syndrome had a significant increase in malondialdehyde levels and the percentage of malondialdehyde release, associated with a marked decrease in GSH concentration, total antioxidant capacity and erythrocyte GSH peroxidase activity compared with controls (154)

The correct sequence of the gene of interest (GOI) was confirmed by the Sanger sequencing service of Microsynth

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