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Periportal assembloids To generate liver periportal assembloids comprising hepatocytes, cholangiocytes and portal fibroblasts, we first prepared the cellular components as follows: nGFP-labelled cholangiocyte organoids (passage 511), grown in cholangiocyte expansion medium (h-CholOrg-EM) as detailed above, were collected from Matrigel using cold AdDMEM/F12 (Invitrogen, 12634010) containing 1% HEPES (ThermoFisher, 15630-056), 1% penicillin/streptomycin (ThermoFisher, 15140-122) and 1% GlutaMAX (ThermoFisher, 35050038)

TIGAR reduces the cellular levels of fructose-2,6-bisphosphate (FRU-2,6-P2), which drives metabolism towards EMP and inhibits gluconeogenesis, thus suppressing EMP and elevating NADPH levels [65]

Van Dyken, S

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