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Targeted Metabolomics on Plasma Metabolite extraction Plasma (20L from 29 female and male samples) was transferred to an individual 1.5 mL microcentrifuge tube and incubated with 400L 80 C 80:20 Methanol (MeOH): H2O extraction solvent on dry ice for 5 minutes post-vortexing

Gastrointestinal adverse events were the most frequently reported, but were predominantly mild to moderate

eLife 11 , e72595 (2022)

#4 Environmental Toxins Exposure to environmental pollutants, heavy metals (such as lead, mercury, and arsenic), pesticides, and other toxins found in air, water, and food sources can impair glutathione synthesis and utilization