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The sequences of primers were as follows: PDGF (5-GAA TCA TAG CTC TCT CCT CGC AC-3 and 5-GAT TCC TCC AAA GCC TCA TAG CAG-3), C-Myc (5-AAT AGA GCT GCT TCG CCT AGA-3 and 5-GAG GTG GTT CAT ACT GAG CAA G-3), TGF- (5-ACC TTG GGC ACT GTT GAA GT-3 and 5-CTC TGG GCT TGT TTC CTC AC-3), MMP2 (5-ACA GCA GGT CTC AGC CTC AT-3 and 5-TGA AGC CAA GCG GTC TAA GT-3), CCN1 (5-CTC CCT GTT TTT GGA ATG GA-3 and 5-TGG TCT TGC TGC ATT TCT TG-3) and GAPDH (5-TGG CAA ATT CCA TGC AC-3 and 5-CCA TGG TGG TGA AGA CGC-3)

While formal clinical guidelines await development, evidence-based principles can guide initial exploration

Option 3: 3ml of bacteriostatic water (lower concentration) Adding 3ml produces a concentration of approximately 1.67mg/ml

123 The distribution of AT 4 binding sites in the human brain has been shown to be clearly different from that of AT 1 receptors