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IBS is also associated with fungal microbiota imbalance, especially overgrowth of Candida ( Akkermansia muciniphila can exacerbate the colonization of Candida tropicalis ( Escherichia coli in the gut significantly affects the hyphae and biofilms formation of Candida , and upregulates pathogenic genes such as ALS3 and HWP1 (Farrokhi et al., 2021)

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The harvested tumors and draining lymph nodes were utilized to prepare single-cell suspensions

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