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Contamination is another formidable risk

These three fish (one pool) were homogenized in 1.7 mL of Tris HCl (pH 7.0, 50 mM) for 1 min in a Potter mixer on an ice bath

CoQH 2 resulting from electron transfer from CI and CII and other metabolic enzymes enters complex III (CIII is the cytochrome bc 1 complex), where it transfers electrons to cytochrome c (cyt c ) and thus becomes reoxidized (FIGURE 2)

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