Here we determined the rate constants for the individual redox reactions between the two redox-sensitive cysteinyl residues of roGFP2 and the active-site cysteinyl residues of the model class I glutaredoxin PfGrx from the malaria parasite Plasmodium falciparum using stopped-flow kinetic measurements in combination with redox mobility shift assays
Chance B, Sies H, Boveris A: Hydroperoxide metabolism in mammalian organs
Apocynin as an antidepressant agent: in vivo behavior and oxidative parameters modulation
Briefly, Huh7 cells were lysed in buffer (150 mM KCl, 25 mM Tris pH 7.4, 5 mM EDTA, 0.5 mM DTT, 0.5% NP-40, 9 g ml 1 leupeptin, 9 g ml 1 pepstatin, 10 g ml 1 chymostatin, 3 g ml 1 aprotinin, 1 mM PMSF and 100 U per ml RNase inhibitor) and centrifuged (12,000 g , 30 min, 4 C)