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For endocytosis, cells were transferred to a 37 C CO 2 tissue culture incubator for 10 min, washed, and fixed

To determine if the increase in enzymatic activity of glutathione peroxidases was due to increased gene expression, qPCR was again conducted to determine if there was any change in abundance of glutathione peroxidase transcripts

The experiment used a completely randomized design with three replications (plastic containers) for each treatment

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