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For fragment [1,3 A - ] ( d,e,f ) and fragment [1,3 A + ] ( g,h,i ) comprising C 4 , the incorporation of an O 18 label is not observed, while fragment [0,2 A-H2O] + ( g,h,i ), including C 3 , contains an O 18 label supporting formation of flavan-3,3,4-triol ( 4 )

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40-42 Among the four cysteine residues found in GSTP1, C47 and C101 have each been demonstrated to be solvent accessible, highly reactive, and targeted by irreversible inhibitors (Figure 5A)