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Results Preliminary phytochemical screening The preliminary phytochemical screening of the EEAF revealed the presence of carbohydrates, alkaloids, flavonoids, tannins, phenolics, saponins, glycosides, steroids, and terpenoids, as indicated by positive results in Table 1

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doi: 10.3389/fbioe.2025.1729166 Received 21 October 2025 Revised 01 December 2025 Accepted 17 December 2025 Published 15 January 2026 Volume 13 - 2025 Edited by Ling Wang, Dalian Medical University, China Reviewed by Nicola Alessio, University of Campania Luigi Vanvitelli, Italy Yesuf Adem Siraj, Bahir Dar University, Ethiopia Updates Copyright 2026 Hu, Li, Hu, Shan, Tang, Jiang, Yi, Chen, Jin, Wang and Wang