Antibodies The following antibodies were used: anti-TRIP12 (Proteintech, 25303, 1:1,000), antip-I-kB (Cell Signaling Technology, 9246 s, 1:1,000), antip-IKK (Cell Signaling Technology, 2078 s, 1:1,000), anti-I-B (Cell Signaling Technology, 4812 s, 1:1,000), antip-p65 (Cell Signaling Technology, 3033, 1:1,000), anti-HA (Cell Signaling Technology, 3724 s, 1:1,000), anti-FLAG (SIGMA, 7425, 1:1,000, or Cell Signaling Technology, 14793 s, 1:1,000), anti-TRAF2 (Abcam, ab-126758, 1:1,000), anti-OTUD5 (Cell Signaling Technology, 20087, 1:1,000), anti-ARMC7 (NOVUS, NBP1-82129, 1:1,000), anti-ZGPAT (Proteintech, 23203, 1:1,000), anti-GTF2B (Proteintech, 16467, 1:1,000), anti-MEAF6 (Themo Fisher Scientific, PA5-40704, 1:1,000), anti-ZBED1 (Proteintech, 26140, 1:1,000), anti-PRDM2 (Proteintech, 27718, 1:1,000), anti-NR2F2 (COUP-TFII) (Cell Signaling Technology, 6434 s, 1:1,000), anti-DHX32 (Proteintech, 68306-1-lg, 1:1,000), anti-Rad18 (Cell Signaling Technology, 9040 s, 1:1,000), anti-Actin (Santa Cruz Biotechnology, sc-47778, 1:1,000), anti-ubiquitin (P4D1, Santa Cruz Biotechnology, sc-8017, 1:1,000), anti-OTUD3 (Proteintech, 29622, 1:1000), anti-OTUD4 (Proteintech, 25070, 1:1000), anti-UBR5 (Santa Cruz Biotechnology, sc-515494, 1:1000), and anti-K48ubiquitin (Abcam, ab-140601, 1:1,000)

HGF is a potent neurotrophic factor in many brain regions (Ebens et al., 1996
Impact of Central and Peripheral TRPV1 and ROS Levels on Proinflammatory Mediators and Nociceptive Behavior
Gatica D, Lahiri V, Klionsky DJ