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Chondrocytes lacking IR grown in micromass culture demonstrated a reduction in Alcian Blue staining that persisted even when cells were cultured in the presence of 10 nmolL 1 insulin, which was partially restored by the treatment of IGF-1 (Figure 3c)

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Concerning Massons trichrome staining, the liver tissue sections of the control and MCP groups exhibited normal morphology of the portal triad consisting of a branch of the portal vein, hepatic artery, and bile duct supported by delicate fibrous tissue