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Oxygen consumption rates An hour before oxygen consumption measurements, cell media was replaced by assay media (2 mM glucose, 0.8 mM Mg 2+ , 1.8 mM Ca 2+ , 143 mM NaCl, 5.4 mM KCl, 0.91 mM NaH 2 PO 4 , and 15 mg/mL Phenol red) for 60 min at 37 C (no CO 2 ) before loading into the Seahorse Bioscience XF96 extracellular analyzer 102

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Ingredients: Aqua, Propylene Glycol, Copper Tripeptide-1, Portulaca Oleracea Extract, Betaine, 1,2-Hexanediol, Xanthan Gum, Sodium Hyaluronate, Hydroxyacetophenone, Gentiana Scabra Extract, Beta-Glucan, Tocopherol

Two cell populations were defined as ischemic coreassociated (ICAM) and ischemic penumbraassociated (IPAM) microglia