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Briefly, cells plated at a density of 2 10 5 cells/cm 2 in 12-well tissue culture-treated polystyrene plates, were first grown for 2 weeks in Williams E medium (Invitrogen, Cergy-Pontoise, France) supplemented with 10% (vol/vol) fetal calf serum (FCS) (Cytiva, Velizy-Villacoublay, France), 100 IU/ml penicillin, 100 g/ml streptomycin, 5 g/ml bovine insulin (SigmaAldrich, Saint-Quentin-Fallavier, France), 2 mM glutamine (Invitrogen) and 50 M hydrocortisone hemisuccinate (Upjohn, Paris La Dfense, France)
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In summary, chrononutrition approaches for older individuals emphasize synchronizing meals with circadian rhythms: eat during daylight and fast at night, maintain regular meal schedules, and incorporate foods that support circadian signals, rich in melatonin or its precursors
Concluding remarks Various strategies can be used to treat hypertension, including lowering the heart rate and cardiac output, dilation of arteries, reducing blood volume, and inhibiting the sympathetic nervous system
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