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In the vehicle controls we observed a significant depletion of sgRNAs targeting commonly accepted core essential genes, suggesting the screen had sensitivity detecting genetic perturbations (Supplementary Fig

IGF1R deficiency decreases proliferation, DNA damage, senescence, and vascularization, attenuates tumor invasion by reduced EMT and fibrosis, and induces apoptosis upon pulmonary metastasis To determine the effect of IGF1R deficiency in the metastatic TME, we immunostained lung tumors of LLC-challenged mice for the following markers: Ki67 (proliferation), 53BP1 (DNA damage), p21 (senescence), C3 (apoptosis), CD31 and CD34 (vascularization), SOX9 (epithelial-mesenchymal transition, EMT), as well as Vimentin, Fibronectin, SMA and Masson (fibrosis)

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Jozefczak M, Remans T, Vangronsveld J, Cuypers A (2012) Glutathione is a key player in metal-induced oxidative stress defenses