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Inconsistent signals across plates are most commonly caused by temperature differences between the plate and reagent at the time of addition (ensure both are equilibrated to room temperature for 30 minutes before mixing), insufficient cell lysis (increase mixing time or speed), or signal read outside the stable window (read plates within 10 minutes to 1 hour after stabilization)

Diabetes 70 , 25452553 (2021)

Targeted brain metabolomics data has been deposited in MassIVE with accession code MSV000095847 [ Targeted plasma metabolomics data has been deposited in MassIVE with accession code MSV000095887 [ Source data are provided with this paper