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The basic formula Concentration (mg/mL) = Total peptide (mg) divided by Total water volume (mL) For a 10mg vial: 10mg divided by 1mL = 10mg/mL 10mg divided by 2mL = 5mg/mL 10mg divided by 3mL = 3.33mg/mL 10mg divided by 5mL = 2mg/mL Calculating your draw volume Draw volume (mL) = Desired dose (mg) divided by Concentration (mg/mL) Example: You want a 4mg dose from a vial reconstituted at 5mg/mL

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Normal dermal fibroblasts were seeded into a 96-well cell culture plate and have been grown to confluence in high glucose media, supplemented with 10% fetal calf serum for around 3 days

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