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Reconstitution technique matters for dosing accuracy

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Cells were seeded into 35 mm dishes (Corning) or 24-well plates (Corning) and allow to grow to confluence in DMEM/F12 medium (Invitrogen 31330038) containing antimycotic/antibiotic (Sigma A5955) and heat-inactivated serum (Gibco 10500064)

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