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Reactions were initiated by the addition of 10 reaction buffer (250 mM Tris-HCl pH 7.5, 500 mM NaCl, 50 mM MgCl, 50 mM ATP, 5 mM DTT) and incubated at 37 C for the specified durations

The Norleual and D-Nle-Tyr-Ile-NH(CH 2 ) 5 CONH 2 samples were precipitated by adding 3 vol of ice-cold acetonitrile (ACN) and the samples were vortexed vigorously

The influence of antibiotic treatment on the behavior and gut microbiome of adult rats neonatally insulted with lipopolysaccharide

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