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ATP levels were determined by measuring fluorescence at Ex/Em = 535/587 nm, using a multi-well plate reader (Synergy H1, BioTek Instruments, Inc., Warsaw, Poland)

Cell viability assays The cells were plated in triplicate in 96-well plates (typically 7000 senescent and 2000 non-senescent cells)

This is the most common question and the answer from a mechanistic standpoint is yes, they are complementary rather than redundant : BPC-157 works through nitric oxide and growth factors (gut-mediated) TB-500 works through actin mobilization and cell migration (structural) These are non-overlapping pathways, which is the theoretical basis for combining them

If you also take blood thinners like Xarelto, always inform your prescribing doctor about all medications you use