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The membrane was washed then probed with the appropriate species-matched secondary horseradish peroxidase conjugated antibody (0.4 mg/mL diluted 1:2500 mouse anti-rabbit IgG (sc-2357, Santa Cruz Biotechnology, Dallas, TX) or 0.4 mg/mL diluted 1:2000 goat anti-mouse IgG (sc-2031)) diluted in 5% skim milk powder in TBST for 1 h at RT on a rocker

Cureus 9 , e1144

In this setting as well, treatments with antioxidant substances could be appropriate, but literature data indicate that antioxidants alone are unable to induce significant clinical changes, except perhaps in mild forms, and must be used in conjunction with standard drug treatments to achieve measurable results (124)

Kidney Disease: Improving Global Outcomes (KDIGO) Diabetes Work Group