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Then 34 10 4 USCs were seeded on the transwell insert, and 100 l and 600 l 50% CM containing 10 mM Y-27632 were added to the apical and basolateral compartments of the transwell, respectively

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Cochlear Decalcification/DissectionFixed cochleae are decalcified in a large volume of 10% EDTA in PBS at room temperature with mild shaking for 72 hours