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Selenite reactivates silenced genes by modifying DNA methylation and histones in prostate cancer cells

On the day of assay, the mannitol and sucrose (MAS) buffer (1) was prepared by diluting sterile-filtered MAS 3 in distilled water, resulting in a solution with the following final concentrations: 220 mM mannitol, 70 mM sucrose, 10 mM KH 2 PO 4 , 5 mM MgCl 2 , 2 mM HEPES, 1 mM EGTA and 0.2% w/v fatty-acid free BSA (3117057001) (pH 7.2), and warmed to 37 C

Derived from a protein found in the stomach, BPC-157 is renowned for its regenerative properties