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Six-to-eight-week-old female DBA/2 mice were purchased from the Jackson Laboratory (Indianapolis, IN) and housed in the LSUHSC-S Animal Resource Facility (four in one cage in microisolators) under standard regulations

Even though the specific roles that NKCC2 in these organs may have in glucose homeostasis remain unexplored, the potential relevance of extrarenal NKCC2 is underlined by the following: i ) the gluconeogenic capacity (Watford, 2005) of the small intestine supplies circulating glucose (Penhoat et al., 2014) and prevents obesity-related hepatic steatosis (Vily-Petit et al., 2020), ii ) the hypothalamus plays a central role in endocrine integration of fuel homeostasis, control of water/energy intake and feeding behavior (Schwartz et al., 2000

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[12] showed that above certain concentrations, selenium can be toxic to yeasts, causing oxidative stress