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Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)
Unmodified peptides with free N-terminal amino groups serve as substrates for aminopeptidases, enzymes that sequentially cleave amino acids from the N-terminus
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