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For the FOXO4-DRI treatment group, 50 M FOXO4-DRI (MCE, HY-P4157) was added to the glucose-free DMEM (Gibco, Catalog #11966-025) medium, followed by 3 h of oxygen-glucose deprivation (OGD) treatment

10.34133/research.0068

cDNA was synthesized from 1 g of purified RNA using HiScript II Q RT SuperMix (Vazyme) and subjected to quantitative real-time PCR (q-PCR) for analyzing the relative mRNA levels of specific genes with proper primers using ChamQ Universal SYBR qPCR Master Mix (Vazyme)

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